In vitro tuberization of yam (Dioscorea sp.): influence of sucrose, light spectra, and plant growth regulators
DOI:
https://doi.org/10.14808/sci.plena.2025.010201Keywords:
micropropagation, LED lamps, carbohydrateAbstract
The effects of different sucrose concentrations, light spectra, and plant growth regulators on in vitro tuberization of yam (Dioscorea sp.) were determined. A completely randomized experimental design was used in a factorial arrangement, with five replications. In the first experiment, three concentrations of sucrose (30, 60, and 90 g.L-1) and four concentrations of naphthalene acetic acid (NAA) (0.0, 1.0, 2.0, and 4.0 mg.L-1) were tested. The highest percentage of tuberization was observed with the use of 90 g.L-1 of sucrose, regardless of the NAA concentrations used. Greater diameter (7.88 mm) and fresh weight (166.13 mg) were obtained by increasing the sucrose concentration to 90 g.L-1. In the second experiment, three LED light spectra (white, blue. and red), two concentrations of NAA (0.0 and 1.0 mg.L‑1), and three concentrations of kinetin (KIN) (0.0, 1.0, and 2.0 mg.L-1) were tested. Tuberization (%) showed higher averages with the use of blue (90.5 %) or red (95.5 %) light spectra or with 1.0 mg.L-1 KIN (91.1%). The culture medium without NAA led to a larger number of microtubers (1.67), as did the use of blue (1.65) or red (1.73) lights, and KIN at concentrations of 1.0 mg.L-1 (1.58) or 2.0 mg.L-1 (1.68). Higher averages of fresh microtuber weight were observed with the use of 0.0 mg.L-1 KIN, 1.0 mg.L-1 NAA, and the blue (276.9 mg) or white (320.4 mg) light spectra. The use of 90 g.L-1 of sucrose, 1 mg.L-1 KIN and 1 mg.L-1 NAA, and the blue-light spectrum increase production of microtubers.
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Copyright (c) 2025 Crislaine Alves dos Santos, Itamara Bomfim Gois, Maria de Fátima Arrigoni-Blank, Lucas de Jesus Santos, Laura Catharine Dória Prata, Taíse Conceição Rodrigues, Arie Fitzgerald Blank

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