Um In vitro viability of pollen grains from genipap accessions
DOI:
https://doi.org/10.14808/sci.plena.2025.010202Keywords:
Genipa americana L., germination, pollen tubeAbstract
The aim of this study was to evaluate the in vitro viability of pollen from genipap accessions at ambient temperature in different culture media. Pollen grains were collected at the Genipap Active Germplasm Bank (BAG Jenipapo) of Embrapa Tabuleiros Costeiros at the Jorge Sobral Experimental Field in the municipality of Nossa Senhora das Dores, Sergipe, Brazil. Three culture media were evaluated in combination with 11 evaluation times and 4 accessions. In vitro viability through germination of the pollen tube using carmine acetic was evaluated in accessions AR2, AR4, CA, and CR3 cultivated in: Lora, Sousa, and modified Sousa medium. A significant effect (p ≤ 0.05) was observed among the interactions of the time after inoculation, culture media, and accession factors regarding pollen germination in vitro. The highest in vitro viability was obtained by accession CR3 in Lora culture medium at 96 h of incubation (86.56%). Regarding pollen viability using carmine acetic 1%, there was no significant difference in viability time for accessions AR2 and CR3. However, a significant difference was observed among the accessions after 168 h, with a decrease in viability for the AR4 accession, to 64.74%. The Lora culture medium is superior for in vitro viability studies of pollen grains by germination of the pollen tube in genipap accessions, and there is a genetic-dependent response for viability of pollen grains from genipap accessions.
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Copyright (c) 2025 Gilmara da Silva Freire, Caroline de Araújo Machado, Ana Veruska Cruz da Silva, Rodolfo de França Alves, Ana da Silva Ledo

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