Biochemical characterization of an exo-polygalacturonase of strain Aspergillus terreus PA3A5T and its application in fruit juice clarification

Géssica Caroline da Silva; Vitória Maciel Delai; Marina Kimiko Kadowaki; Rita de Cássia Garcia Simão; José Luis da Conceição Silva; Alexandre Maller

doi:10.14808/sci.plena.2024.071101

Biochemical characterization of an exo-polygalacturonase of strain Aspergillus terreus PA3A5T and its application in fruit juice clarification

Authors

Géssica Caroline da Silva Centro de Ciências Médicas e Farmacêuticas/Laboratório de Bioquímica de Microrganismos, Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0002-6704-7415
Vitória Maciel Delai Centro de Ciências Médicas e Farmacêuticas/Laboratório de Bioquímica de Microrganismos, Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0003-2682-1248
Marina Kimiko Kadowaki Centro de Ciências Médicas e Farmacêuticas/Laboratório de Bioquímica de Microrganismos, Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0002-9174-5811
Rita de Cássia Garcia Simão Centro de Ciências Médicas e Farmacêuticas/Laboratório de Bioquímica de Microrganismos, Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0003-2999-1218
José Luis da Conceição Silva Centro de Ciências Médicas e Farmacêuticas/Laboratório de Bioquímica de Microrganismos, Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0002-5460-8797
Alexandre Maller Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0002-8065-9184

DOI:

https://doi.org/10.14808/sci.plena.2024.071101

Keywords:

pectinase, microbial enzymes, filamentous fungi

Abstract

This study aimed to characterize an exo-PG from Aspergillus terreus PA3A5T and evaluate its application in the clarification of fruit juices. Exo-PG was obtained by submerged fermentation and it was purified by ion-exchange chromatography and gel filtration. The enzyme presented molecular mass of 95.2 kDa, with maximum activity at 70 °C in a pH of 4.0 and remaining stable in an acid pH at 60°C. FeCl₂, SnCl₂, CoCl₂, ZnSO₄, NaCl, Al₂(SO4)₃, MgCl₂, MnCl₂, and CuSO₄ ions activated the enzymatic activity, and PbCl₂ inhibited it completely. The value of Km was 1.83 mg mL^-1 and Vmax was 335 U mg^-1. The A. terreus PA3A5T exo-PG proved to be very effective in the clarification process of the juices, significantly reducing viscosity and color.

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Published

2024-08-12

How to Cite

da Silva, G. C., Delai, V. M., Kadowaki, M. K., Simão, R. de C. G., Silva, J. L. da C., & Maller, A. (2024). Biochemical characterization of an exo-polygalacturonase of strain Aspergillus terreus PA3A5T and its application in fruit juice clarification . Scientia Plena, 20(7). https://doi.org/10.14808/sci.plena.2024.071101

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Issue

Vol. 20 No. 7 (2024): Julho/July 2024

Section

Articles

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