Artificial inoculation of Fusarium oxysporum in seeds of Phaseolus vulgaris
DOI:
https://doi.org/10.14808/sci.plena.2016.070202Keywords:
bean, fusarium wilt, inoculation methods.Abstract
Several diseases that affect the bean crop are transmitted by seeds for such studies are important to infected seeds that produce viable seedlings. Thus, the objective of this study was to test different Fusarium oxysporum inoculation methods in bean seeds and its effect on seedling development. They were used seeds of the cv. Expedito BRS, which were inoculated by the spores suspension methods, water contact constraint and inoculating the substrate with BDA discs containing mycelia. The experimental design was completely randomized with eight repetitions. They were made emergency assessments, length and dry mass of shoots and incidence of symptoms of the disease in stem and leaf. Inoculation of the pathogen Fusarium oxysporum in bean seeds negatively affects the development of seedlings. The spore suspension method produces symptoms of the disease with the least impairment in the development of seedlings.
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