Validation of a UV-VIS Spectrophotometric method for the determination of usnic acid /collagen-based membranes

Autores

  • Paula Santos Nunes Universidade Federal de Sergipe
  • Douglas Costa Jesus
  • Marília Santos Bezerra
  • Jamille Cristina Campos Souza
  • Francilene Amaral Silva
  • Mairim Russo Serafini
  • Bruno Santos Lima
  • Saravanan Shanmugam
  • Ricardo Luiz Cavalcanti Albuquerque Junior
  • Adriano Antunes de Souza Araújo

DOI:

https://doi.org/10.14808/sci.plena.2015.094501

Palavras-chave:

Usnic acid, Collagen membranes, UV Spectrophotometry.

Resumo

The usnic acid (UA) is a secondary metabolite of lichen that has shown potential pharmacological effects

such as antimicrobial, antiviral, antiproliferative and anti-inflammatory activities. Collagen is one of the most useful biomaterials with various applications as drug delivery systems. The aim of this study was to develop and validate a quantitative UV spectrophotometric method for the determination of UA levels in collagen membranes. The validation parameters were assessed according to the Brazilian Health Surveillance Agency (ANVISA). The specificity revealed that the excipients in the formulation did not interfere with the analysis. The linearity in the range of 2-10 μg/mL presented a correlation coefficient of 0.9994. The method showed excellent repeatability (Relative Standard Deviation (RSD) < 1.0%). The accuracy revealed a mean percentage recovery of 100.43%. The method was robust for the variation of temperature and solvent. The detection and quantization limits were found at 0.109 and 0.364 µg/mL. The total rate recovery from the analyzed membranes showed values between 100.4 and 83.2%.  

Biografia do Autor

Paula Santos Nunes, Universidade Federal de Sergipe

Departamento de Morfologia

Ciências da Saúde

Douglas Costa Jesus

Departamento de Farmácia

Ciências da Saúde

Marília Santos Bezerra

Departamento de Farmácia

Ciências da Saúde

Jamille Cristina Campos Souza

Departamento de Farmácia

Ciências da Saúde

Francilene Amaral Silva

Departamento de Farmácia

Ciências da Saúde

Mairim Russo Serafini

Departamento de Farmácia

Ciências da Saúde

Bruno Santos Lima

Departamento de Farmácia

Ciências da Saúde

Saravanan Shanmugam

Departamento de Farmácia

Ciências da Saúde

Ricardo Luiz Cavalcanti Albuquerque Junior

Instituto de Tecnologia e Pesquisa

Ciências da Saúde

Adriano Antunes de Souza Araújo

Departamento de Farmácia

Ciências da Saúde

Downloads

Publicado

2015-09-06