Biochemical studies of the bromelain enzimatic activity of the enzyme pineapple Ananas comusus

Authors

  • A. França-Santos Laboratório de Enzimologia – Departamento de Fisiologia, Universidade Federal de Sergipe
  • R. S. Alves Laboratório de Enzimologia – Departamento de Fisiologia, Universidade Federal de Sergipe
  • N. S. Leite Laboratório de Enzimologia – Departamento de Fisiologia, Universidade Federal de Sergipe
  • R. P. M. Fernandes Laboratório de Enzimologia – Departamento de Fisiologia, Universidade Federal de Sergipe

Keywords:

biochemical characterization, plant protease, pineapple, Kunitz, cv Pérola

Abstract

This study aimed to determine bromelain enzimatic activity of the enzyme pineapple Ananas comusus cv. Pérola in both in vivo and in natura conditions and partial biochemical characterization of this enzyme that is widely used in pharmaceutical and food industries. The proteolytic activity was measured by digestion of casein by the Kunitz (1947) method. Higher activity was observed for bromelain extracted from pineapple fruits when compared to leaves of pineapple cultured  in natura under hydroponic condition. The variation in proteolytic activity due to pH and temperature was determined at pHs between 4.0 to 9.0, and temperatures between 20ºC to 70°C. Bromelain showed optimum pH of action 5.0 in sodium acetate buffer and optimum temperature of 40ºC.

Downloads

How to Cite

França-Santos, A., Alves, R. S., Leite, N. S., & Fernandes, R. P. M. (2011). Biochemical studies of the bromelain enzimatic activity of the enzyme pineapple Ananas comusus. Scientia Plena, 5(11). Retrieved from https://scientiaplena.emnuvens.com.br/sp/article/view/749

Issue

Vol. 5 No. 11 (2009): November/Novembro 2009

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work

Most read articles by the same author(s)