Toxicidade de inseticida agrícola em bioensaio com Artemia salina

Eliane Alves Lustosa; Marcos Antonio Nobrega de Sousa; Thaís Lucena de Oliveira; Jeniffer  Gabrielly de Sousa Pereira; Francisca Vitória Amaral Nóbrega

doi:10.14808/sci.plena.2023.055201

Authors

Eliane Alves Lustosa Universidade Federal de Campina Grande
Marcos Antonio Nobrega de Sousa Federal University of Campina Grande https://orcid.org/0000-0001-6550-6609
Thais Lucena de Oliveira Federal University of Campina Grande https://orcid.org/0000-0002-6484-6713
Jeniffer Gabrielly de Sousa Pereira Federal University of Campina Grande https://orcid.org/0000-0003-2696-3027
Possui formação na área da construção civil, com o curso Técnico em Edificações, pelo Instituto Federal da Paraíba. Atualmente, está em formação, no curso de Licenciatura em Ciências Biológicas, pela Universidade Federal de Campina Grande- CSTR UNIFIP – University Center of Patos, Brazil https://orcid.org/0000-0002-6480-1240

DOI:

https://doi.org/10.14808/sci.plena.2023.055201

Keywords:

Pesticides, Cyromazine, Toxicology

Abstract

Insecticides are pesticides used to control insects that are harmful to agricultural crops. However, the excessive use of pesticides may result in toxic effects on human health and numerous socio-environmental impacts. Different toxicity assays can be used to analyze the toxic effect of certain substances. Among them, the Artemia salina bioassay is considered a rapid, reliable, and cost-effective test when compared to tests on other animal models, such as mammals. The objective of this study was to evaluate the toxic potential of the agricultural insecticide cyromazine using the microcrustacean A. salina. The nauplii remained in contact with the test substance diluted in saline water solution, in decreasing concentrations from the usual dose, and the negative control occurred only in saline solution. The experiment was performed in triplicate, and after 24 and 48 hours of exposure, the surviving larvae were counted, and acute toxicity, the median lethal concentration (CL50), and the dose-response relationship were calculated through statistical analysis. The insecticide was considered toxic, and acute toxicity was higher after 48 hours of exposure. Additionally, a dose-response relationship was observed between the concentrations and the mortality of the exposed organisms. Thus, it is important to emphasize that monitoring the toxic effects of this insecticide is essential, considering that it is a widely used product in the agricultural industry.

Author Biographies

Marcos Antonio Nobrega de Sousa, Federal University of Campina Grande

Graduated in Biological Sciences from the Federal University of Paraíba, master's degree in Biological Sciences (Zoology) from the Federal University of Paraíba and PhD in Biological Sciences (Biology - Genetics) from the University of São Paulo. Professor at the Federal University of Campina Grande. He has experience in the area of Genetics, working mainly on the following topics: Cytogenetics, Mutagenesis, Biological Evolution, Education, Molecular Biology and Bioinformatics.

Thais Lucena de Oliveira, Federal University of Campina Grande

He has secondary-high school education at Colégio Cristo Rei (2016). He is currently a scientific initiation at the Federal University of Campina Grande and a member of the Academic League of Genetics at the Federal University of Goiás. He has experience in the area of Genetics, with emphasis on Animal Genetics.

Jeniffer Gabrielly de Sousa Pereira, Federal University of Campina Grande

He has training in the area of civil construction, with the Technical course in Buildings, by the Federal Institute of Paraíba. Currently, he is in formation, in the course of Degree in Biological Sciences, at the Federal University of Campina Grande - CSTR

Possui formação na área da construção civil, com o curso Técnico em Edificações, pelo Instituto Federal da Paraíba. Atualmente, está em formação, no curso de Licenciatura em Ciências Biológicas, pela Universidade Federal de Campina Grande- CSTR, UNIFIP – University Center of Patos, Brazil

Bachelor and Degree in Biological Sciences from the State University of Paraíba - UEPB (2003). Bachelor of Laws from Faculdades Integradas de Patos - FIP (2018). Specialist in Environmental Management (2010) and Specialist in Labor and Social Security Law (2022). With experience in the areas of Law and Environmental Sciences.