Irradiated peripheral blood mononuclear cells for assessing individual radiosensitivity in laryngeal cancer radiotherapy

Authors

  • Ademir Amaral Departamento de Energia Nuclear-UFPE
  • Heberton Ferreira Departamento de Radioterapia - Instituto de Medicina Integral Professor Fernando Figueira
  • Thaisa Feliciano de Souza Departamento de Energia Nuclear - Universidade Federal de Pernambuco
  • Jonathan Melo Departamento de Radioterapia- Real Hospital Português
  • Amanda Iumatti Santos Firmo Xavier Departamento de Energia Nuclear - Universidade Federal de Pernambuco
  • Edvane Borges da Silva Departamento de Energia Nuclear - universidade Federal de Pernambuco
  • Luciano Rodolfo Ferreira de Lucena Departamento de Energia Nuclear - Universidade Federal de Pernambuco
  • André Maciel Netto Departamento de Energia Nuclear - Universidade Federal de Pernambuco

DOI:

https://doi.org/10.14808/sci.plena.2023.077501

Keywords:

laryngeal cancer, radiotherapy, radiosensitivity

Abstract

In laryngeal cancer radiotherapy (LCRT), dermatitis, dysphagia, and odynophagia are common acute side effects, varying in intensity depending mainly on the patient's radiosensitivity. This study investigated the survival rate of in vitro irradiated peripheral blood mononuclear cells (PBMC) as a pre-therapeutic test to assess individual radiosensitivity and predict the degree of these acute side effects in LCRT. 16 patients diagnosed with early-stage laryngeal cancer undergoing radiotherapy without adjuvant chemotherapy participated as volunteers in this study. Before the beginning of treatment, a sample of PBMC was collected and irradiated with 2.5 Gy using a 6 MV linear accelerator. After 72-h cell incubation, the trypan blue exclusion test was performed to score viable cells through optical microscopy. The percentages of cell viability were compared to the intensity of acute side effects experienced by LCRT patients. Even though no correlation was found regarding the observed degrees of radiation dermatitis, our results indicate that patients with survival rates of in vitro irradiated PBMC higher than 70% are more likely to experience moderate to severe acute odynophagia and dysphagia after LCRT. The proposed and discussed methodology points to a practical and affordable pre-therapeutic test to evaluate individual radiosensitivity in order to prevent or reduce acute side effects in laryngeal cancer radiotherapy.

Downloads

Published

2023-08-21

How to Cite

Amaral, A., Ferreira, H., Feliciano de Souza, T. ., Melo, J. ., Iumatti Santos Firmo Xavier, A. ., Borges da Silva, E. ., Rodolfo Ferreira de Lucena, L. ., & Maciel Netto, A. (2023). Irradiated peripheral blood mononuclear cells for assessing individual radiosensitivity in laryngeal cancer radiotherapy . Scientia Plena, 19(7). https://doi.org/10.14808/sci.plena.2023.077501

Issue

Vol. 19 No. 7 (2023): Julho/July

Section

Articles

License

Copyright (c) 2023 Ademir Amaral, Heberton Ferreira, Thaisa Feliciano de Souza, Jonathan Melo, Amanda Iumatti Santos Firmo Xavier, Edvane Borges da Silva, Luciano Rodolfo Ferreira de Lucena, André Maciel Netto

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work