Saccharomyces cerevisiae as a model for teaching toxicology to high school students
DOI:
https://doi.org/10.14808/sci.plena.2021.012702Keywords:
toxicity, yeasts, bioindicatorsAbstract
The yeast Saccharomyces cerevisiae is commonly used in toxicity bioassays because it is a eukaryotic model that is easily accessible and cultivated. Thus, this work proposes an experimental protocol of easy replication, using this yeast and chemicals easily acquired on the market, to enable the teacher to introduce, in a practical way, concepts of toxicology for high school students. Therefore, the mortality percentage of this yeast was evaluated in the presence of commercial formulations of ethanol and H2O2 and a linear behavior of mortality was observed with the gradual increase in the concentration of chemicals. Superior peroxide toxicity was verified, possibly associated with oxidative stress. This protocol allows the teacher to demonstrate that toxicity is closely related to the concentration of chemicals and opens up prospects for testing with other samples, biological, such as plant extracts or chemicals such as wastewater, pesticides and others.
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Copyright (c) 2021 Luciana Cristina Vitorino, Lígia Gabriela de Sá Vanin, Layara Alexandre Bessa, Rodrigo Braghiroli
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