Use of yeasts for biomass production in forage palm extract (Opuntia ficus-indica)

Authors

  • Clarissa Izabel Matos Lins Center of Biosciences / Mycology of Department , Federal University of Pernambuco (UFPE), 50670-420, Recife-PE, Brazil.
  • Flávio Fonseca Veras Institute of Food Science and Technology / Laboratory of Biochemistry and Applied Microbiology, Federal University of Rio Grande do Sul (UFRGS), 91501-970, Porto Alegre-RS, Brazil
  • Danyelly Bruneska Gondim Martins Immunopathology Laboratory Keizo Asami / Department of Biochemistry, Federal University of Pernambuco (UFPE), 50670-901, Recife-PE, Brazil
  • José Luiz De Lima Filho Immunopathology Laboratory Keizo Asami / Department of Biochemistry, Federal University of Pernambuco (UFPE), 50670-901, Recife-PE, Brazil

DOI:

https://doi.org/10.14808/sci.plena.2018.056201

Keywords:

Candida spp., cactus, fermentation

Abstract

Opuntia ficus-indica can be applied in bioproduct production because of its composition rich in carbohydrates. In this work, the liquid extract from stem structure (cladodes) were used for the selection of yeast with biotechnological interest. Candida utilis, C. lipolytica, Saccharomyces cerevisiae and Kluyveromyces marxianus were cultivated in this extract obtained after cactus treatment. Microbial growth aliquots were collected for analysis of biomass, sugars, pH and kinetic parameters. K. marxianus showed higher growth rate (μ= 0.25h-1) and higher percentage of consumption of sugar (95.68%). C. utilis obtained better biomass yield on the substrate consumed (Yx/s= 2.69). C. lipolytica was highlighted by showing a better kinetic profile, since it presented higher production of biomass (ΔXmax= 10.93 g/l) and productivity within 24 hours of cultivation (P= 0.58 g/l.h-1) on the other yeasts tested. The influence of different salt concentrations in the growth of C. lipolytica in O. ficus-indica extract was tested with NaCl and KCl (0.1M, 0.25M, 0.5M, 1M, 2M and 4M). The growth under stress C. lipolytica was tolerant to saline concentrations (up to 1M NaCl and 0.5 M for KCl). The higher concentrations inhibited the cell growth. In the concentration of 4M was considered lethal to cells and sub-lethal 2M. Candida lipolytica cells might be able to adapt to osmotic stress by the concentration of 1M. The results suggest the use of this cactus specie as an alternative substrate for the microbial biomass production.

Author Biographies

Clarissa Izabel Matos Lins, Center of Biosciences / Mycology of Department , Federal University of Pernambuco (UFPE), 50670-420, Recife-PE, Brazil.

http://lattes.cnpq.br/8122871504635442

Flávio Fonseca Veras, Institute of Food Science and Technology / Laboratory of Biochemistry and Applied Microbiology, Federal University of Rio Grande do Sul (UFRGS), 91501-970, Porto Alegre-RS, Brazil

http://lattes.cnpq.br/8126172449861016

Danyelly Bruneska Gondim Martins, Immunopathology Laboratory Keizo Asami / Department of Biochemistry, Federal University of Pernambuco (UFPE), 50670-901, Recife-PE, Brazil

http://lattes.cnpq.br/5510193262532567

José Luiz De Lima Filho, Immunopathology Laboratory Keizo Asami / Department of Biochemistry, Federal University of Pernambuco (UFPE), 50670-901, Recife-PE, Brazil

 http://lattes.cnpq.br/2834403735297272

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Published

2018-06-12

How to Cite

Matos Lins, C. I., Veras, F. F., Gondim Martins, D. B., & De Lima Filho, J. L. (2018). Use of yeasts for biomass production in forage palm extract (Opuntia ficus-indica). Scientia Plena, 14(5). https://doi.org/10.14808/sci.plena.2018.056201

Issue

Vol. 14 No. 5 (2018): May/Maio 2018

Section

Articles

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