Enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia)

Autores

DOI:

https://doi.org/10.14808/sci.plena.2020.051501

Palavras-chave:

Byrsonima crassifolia, phenolic compounds, Response surface methodology

Resumo

The aim of this work was to evaluate the enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia) employing response surface methodology (RSM), in order to determine the best extraction conditions. The chemical composition of the leaves and characteristics of the enzymes were determined. The independent variables were temperature (19 to 70°C), pH (2.4 to 7.5) and reaction time (19 to 220 minutes). A conventional and optimized triple extraction with organic solvents was used as control. As result to solvent extraction was observed 73.28 mg of gallic acid equivalents (GAE) g-1 DM, while under the best condition for enzyme-assisted extraction (temperature: 60°C; pH: 4.5; and time: 220 min), the concentration was 90.65 mg GAE g-1. The independent variables significant were: temperature, pH and log time at the linear level and temperature at the quadratic level. A similar methodology could be encouraged for other natural products rich in phenolic compounds.

Biografia do Autor

Johnatt Allan Rocha Oliveira, Universidade Federal do Pará

Professor adjunto na Faculdade de Nutrição, Instituto de Ciências da Saúde, Universidade Federal do Pará

Andrea Komesu, Universidade Estadual de Campinas

Faculdade de Engenharia Química

Luiza Helena Da Silva Martins, Universidade Federal do Pará

Faculdade de Engenharia de Alimentos

Hervé Rogez, Universidade Federal do Pará

Instituto de Ciências Biológicas, Faculdade de Biotecnologia

Rosinelson da Silva Pena, Universidade Federal do Pará

Faculdade de Engenharia de Alimentos, Instituto de Tecnologia

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Publicado

2020-06-23

Como Citar

Oliveira, J. A. R., Komesu, A., Martins, L. H. D. S., Rogez, H., & Pena, R. da S. (2020). Enzyme-assisted extraction of phenolic compounds from murucizeiro leaves (Byrsonima crassifolia). Scientia Plena, 16(5). https://doi.org/10.14808/sci.plena.2020.051501

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